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Sökning: WFRF:(Fuentes Christian) > (2012)

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  • Klionsky, Daniel J, et al. (författare)
  • Guidelines for the use and interpretation of assays for monitoring autophagy
  • 2012
  • Ingår i: Autophagy. - Landes Bioscience. - 1554-8635. ; 8:4, s. 445-544
  • Forskningsöversikt (refereegranskat)abstract
    • In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
  • Fuentes, Christian (författare)
  • Green Marketing at the Store: The socio-material life of a t-shirt
  • 2012
  • Annan publikation (övrigt vetenskapligt)abstract
    • Green products are becoming part of contemporary consumer cultures and part of everyday life. But how are green products constructed? And how do they work? This paper is concerned with the role that marketing plays in the making and circulation of green products. My aim is to illuminate the process of green making. Departing from an understanding of marketing as socio- material practice I analyse how a green outdoor product - a t-shirt - was constructed as green through the marketing practices of the Nordic Nature Shops. Focusing on this retail corporation and examining the practices of trail making, attending and selling I suggest that these t-shirts become green through a process of socio-material inscription. Through marketing practices green moral is generated and linked to the t-shirts giving and potentially making them desirable consumption objects to be used in the construction of consumers green identities. But, this process of green making is, I argue, a difficult accomplishment with ambiguous outcomes. The greening of consumer culture is not an easy accomplishment and commercial greening might also have a number of negative societal consequences.
  • Fuentes, Christian (författare)
  • Socio-Cultural Retailing: A literature review
  • 2012
  • Annan publikation (övrigt vetenskapligt)abstract
    • The aim of this paper is to contribute to the cultural turn in retail studies by offering an overview of the interdisciplinary field of socio-cultural retailing and, based on this introduction, draw out and discuss what this body of work can contribute to the field of retail marketing. The paper reviews socio- cultural retail research from the disciplines of marketing, cultural geography, sociology and anthropology. Taken together this body of work clearly shows that retailing and shopping practices are anchored in and also work to reproduce wider social and cultural processes. Retailing does not exist part from socio-cultural processes of identity and meaning construction but is intrinsically interlinked with these processes. Therefore, to understand why some store concepts, marketing practices and products work and others fail; it is important to understand the socio-cultural processes underlying the practices of retailing, shopping and consumption. In addition, accepting that retailing practices and spaces are not only linked to socio-cultural processes but also actively work to reproduce these processes means acknowledging the political role of retailing. From this perspective, retail practices and spaces are actively involved in the construction of identities, meanings and worldviews; they shape, to some extent, the way we view the world and ourselves.
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