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  • Abdelraheem, Mohamed Ahmed, et al. (författare)
  • Executing Boolean queries on an encrypted Bitmap index
  • 2016
  • Ingår i: CCSW 2016 - Proceedings of the 2016 ACM Cloud Computing Security Workshop, co-located with CCS 2016. - Association for Computing Machinery (ACM). - 9781450345729 ; s. 11-22
  • Konferensbidrag (refereegranskat)abstract
    • We propose a simple and efficient searchable symmetric encryption scheme based on a Bitmap index that evaluates Boolean queries. Our scheme provides a practical solution in settings where communications and computations are very constrained as it offers a suitable trade-off between privacy and performance.
  • Ahlqvist, Josefin, et al. (författare)
  • Affinity binding of inclusion bodies on supermacroporous monolithic cryogels using labeling with specific antibodies
  • 2006
  • Ingår i: Journal of Biotechnology. - Elsevier. - 1873-4863. ; 122:2, s. 216-225
  • Tidskriftsartikel (refereegranskat)abstract
    • A new chromatographic method based on affinity supermacroporous monolithic cryogels is developed for binding and analyzing inclusion bodies during fermentation. The work demonstrated that it is possible to bind specific IgG and IgY antibodies to the 15 and 17 amino acids at the terminus ends of a 33 kDa target protein aggregated as inclusion bodies. The antibody treated inclusion bodies from lysed fermentation broth can be specifically retained in protein A and pseudo-biospecific ligand sulfamethazine modified supermacroporous cryogels. The degree of binding of IgG and IgY treated inclusion bodies to the Protein A and sulfamethazine gels are investigated, as well as the influence of pH on the sulfamethazine ligand. Optimum binding of 78 and 72% was observed on both protein A and sulfamethazine modified cryogel columns, respectively, using IgG labeling of the inclusion bodies. The antibody treated inclusion bodies pass through unretained in the sulfamethazine supermacroporous gel at pH that does not favour the binding between the ligand on the gel and the antibodies on the surface of inclusion bodies. Also the unlabeled inclusion bodies went through the gel unretained, showing no non-specific binding or trapping within the gel. These findings may very well be the foundation for the building of a powerful analytical tool during fermentation of inclusion bodies as well as a convenient way to purify them from fermentation broth. These results also support our earlier findings [Kumar, A., Plieva, F.M., Galaev, I.Yu., Mattiasson, B.. 2003. Affinity fractionation of lymphocytes using a monolithic cyogel. J. Immunol. Methods 283, 185-194] with mammalian cells that were surface labeled with specific antibodies and recognized on protein A supermacroporous gels. A general binding and separation system can be established on antibody binding cryogel affinity matrices.
  • Ahlqvist, J., et al. (författare)
  • Monitoring the production of inclusion bodies during fermentation and enzyme-linked immunosorbent assay analysis of intact inclusion bodies using cryogel minicolumn plates
  • 2006
  • Ingår i: Analytical Biochemistry. - Elsevier. - 1096-0309. ; 354:2, s. 229-237
  • Tidskriftsartikel (refereegranskat)abstract
    • A novel minicolumn chromatgraphic method to monitor the production of inclusion bodies during fermentation and anenzyme-linked immunosorbent assay (ELISA) system allowing direct analysis of the particles with surface-displayed antigens are described. A 33-kDa protein containing 306 amino acids with three sulfur bridges produced its inclusion bodies wits labeled with polyclonal antibodies against 15 amino acid (anti-A15) and 17 amino acid (anti-B17) residues at the N- and C-terminal ends of the protein, respectively. Labeled particles were bound to macroporous Monolithic protein A-cryogel adsorbents inserted into the open-ended wells of a 96-well plate (referred to as protein A-cryogel minicolumn plate). The concept behind this application is that the binding degree of inclusion bodies from lysed fermentation broth to the cryogel minicolumns increases with an increase in their concentration during fermentation. The technique allowed LIS to monitor the increase in the production levels of the inclusion bodies as the fermentation process progressed. The system also has a built-in quality parameter to ensure that the target protein has been fully expressed. Alternatively, inclusion bodies immobilized on phenyl-cryogel minicolumn plate were used in indirect ELISA based on anti-A15 and anti-B17 antibodies against terminal amino acid residues displayed oil the surface of inclusion bodies. Drainage-protected properties of the cryogel minicolumns allow performance of successive reactions with tested immunoglobulin G (IgG) samples and enzyme-conjugated secondary I-G and of enzymatic reaction within the adsorbent. (c) 2006 Elsevier Inc. All rights reserved.
  • Ahlström, Johan, et al. (författare)
  • Galvanic corrosion properties of steel in water saturated concrete
  • 2015
  • Ingår i: Materials and Corrosion - Werkstoffe und Korrosion. - John Wiley & Sons. - 1521-4176. ; 66:1, s. 67-75
  • Tidskriftsartikel (refereegranskat)abstract
    • Aluminum-based sacrificial anodes were installed to reinforced concrete to stop ongoing corrosion in cooling water tunnels in a Swedish nuclear power plant. The steel rebars were also unintentionally connected to stainless steel water pumps. Therefore, the consumption rate of the sacrificial anodes was higher than predicted. An experimental and a field study were performed to assess if the steel rebar suffer from galvanic corrosion and if the stainless steel pumps are responsible alone for the high consumption rate. It was found from the experimental study that there is an increased risk of galvanic corrosion for steel rebar when the corrosion potential is raised to -200mV (SCE) for samples with 1% Cl- by mass of cement and -500mV (SCE) for samples with 2% Cl- by mass of cement. The experimental results were compared with the corrosion potential measured in the cooling water tunnel where sacrificial anodes were in use and not mounted at all. The cooling water tunnel without sacrificial anodes had generally more anodic corrosion potentials compared to the tunnel with anodes. The tunnel with anodes had also more anodic potentials closer to the stainless steel pumps than further away which means that the rebar is affected by the stainless steel pumps. However, the measured corrosion potentials in the tunnels were not as anodic as the potentials needed for high galvanic current measured in the experimental study.
  • Albèr, C., et al. (författare)
  • Effects of water gradients and use of urea on skin ultrastructure evaluated by confocal Raman microspectroscopy
  • 2013
  • Ingår i: Biochimica et Biophysica Acta - Biomembranes. - Elsevier. - 0005-2736. ; 1828:11, s. 2470-2478
  • Tidskriftsartikel (refereegranskat)abstract
    • The rather thin outermost layer of the mammalian skin, stratum corneum (SC), is a complex biomembrane which separates the water rich inside of the body from the dry outside. The skin surface can be exposed to rather extreme variations in ambient conditions (e.g. water activity, temperature and pH), with potential effects on the barrier function. Increased understanding of how the barrier is affected by such changes is highly relevant for regulation of transdermal uptake of exogenous chemicals. In the present study we investigate the effect of hydration and the use of a well-known humectant, urea, on skin barrier ultrastructure by means of confocal Raman microspectroscopy. We also perform dynamic vapor sorption (DVS) microbalance measurements to examine the water uptake capacity of SC pretreated with urea. Based on novel Raman images, constructed from 2D spectral maps, we can distinguish large water inclusions within the skin membrane exceeding the size of fully hydrated corneocytes. We show that these inclusions contain water with spectral properties similar to that of bulk water. The results furthermore show that the ambient water activity has an important impact on the formation of these water inclusions as well as on the hydration profile across the membrane. Urea significantly increases the water uptake when present in skin, as compared to skin without urea, and it promotes formation of larger water inclusions in the tissue. The results confirm that urea can be used as a humectant to increase skin hydration.
  • An, Junxue, et al. (författare)
  • Tethered Poly(2-isopropyl-2-oxazoline) Chains : Temperature Effects on Layer Structure and Interactions Probed by AFM Experiments and Modeling
  • 2015
  • Ingår i: Langmuir. - 0743-7463. ; 31:10, s. 3039-3048
  • Tidskriftsartikel (refereegranskat)abstract
    • Thermoresponsive polymer layers on silica surfaces have been obtained by utilizing electrostatically driven adsorption of a cationic-nonionic diblock copolymer. The cationic block provides strong anchoring to the surface for the nonionic block of poly(2-isopropyl-2-oxazoline), referred to as PIPOZ. The PIPOZ chain interacts favorably with water at low temperatures, but above 46 degrees C aqueous solutions of PIPOZ phase separate as water becomes a poor solvent for the polymer. We explore how a change in solvent condition affects interactions between such adsorbed layers and report temperature effects on both normal forces and friction forces. To gain further insight, we utilize self-consistent lattice mean-field theory to follow how changes in temperature affect the polymer segment density distributions and to calculate surface force curves. We find that with worsening of the solvent condition an attraction develops between the adsorbed PIPOZ layers, and this observation is in good agreement with predictions of the mean-field theory. The modeling also demonstrates that the segment density profile and the degree of chain interpenetration under a given load between two PIPOZ-coated surfaces rise significantly with increasing temperature.
  • Andersson, Berit, et al. (författare)
  • Experimental study of thermal breakdown products from halogenated extinguishing agents
  • 2011
  • Ingår i: Fire Safety Journal. - Elsevier. - 0379-7112. ; 46:3, s. 104-115
  • Tidskriftsartikel (refereegranskat)abstract
    • Thermal breakdown products from four different fire extinguishing agents have been analysed. The agents studied were: bromotrifluoromethane (Halon 1301), pentafluoroethane (HFC 125), heptafluoropropane (HFC 227ea) and dodecafluoro-2-methyl-pentane-3-one (C6F-ketone). In the tests, the studied agent was introduced into a propane flame in a diffusion flame burner. The combustion products were analysed using both conventional IR-techniques and FTIR. It could be concluded that for all four extinguishing agents that the extinguishing agent takes part in the combustion process resulting in production of CO and CO2, which was also confirmed by the increase in smoke production with increasing amounts of agent introduced into the flame. Production of HF and COF2 was found in experiments with all four extinguishing agents. In experiments with Halon 1301, the production of HBr was also determined. It was found that the main fraction of fluorine ends up as HF for HFC 227ea and HFC 125 at lower relative application rates. When approaching extinguishing concentrations, the fraction recovered as COF2 increases, but the total recovery of fluorine, including HF and the remaining part of the fluorine, is found in various organic breakdown products. The recovery of fluorine as HF and COF2 for the C6F-ketone is lower compared to HFC 227ea and HFC 125. There is a clear difference in the recovery of fluorine for Halon 1301 where an almost quantitative recovery as HF or COF2 is found. Halon 1301 additionally contains bromine, which, to a large extent, is recovered as HBr.
  • Andersson, Berit, et al. (författare)
  • Thermal breakdown of extinguishing agents
  • 2008
  • Rapport (övrigt vetenskapligt)abstract
    • Four fire extinguishing agents has been investigated, bromotrifluoromethane, Halon 1301, pentafluoroethane, HFC 125, heptafluoropropane, HFC 227ea and dodecafluoro-2- methylpentane-3-one, C6F-ketone. The inerting concentrations were determined in a cubic pressure vessel with a volume of 8 litres. A cup burner was used to find the flameextinguishing concentrations for the agents. Thermal breakdown was also studied and for this purpose the studied agent was introduced into a flame of propane in a tubular burner. The produced combustion products were analysed both with conventional IR-techniques and with FTIR. It was found that HF and COF2 were produced from all studied agents. Models and theories on the mechanisms of the breakdown processes are presented.
  • Andersson, M., et al. (författare)
  • Toward an enzyme-based oxygen scavenging laminate. Influence of industrial lamination conditions on the performance of glucose oxidase
  • 2002
  • Ingår i: Biotechnology and Bioengineering. - John Wiley & Sons. - 1097-0290. ; 79:1, s. 37-42
  • Tidskriftsartikel (refereegranskat)abstract
    • The laminate consisted of several polymer layers, aluminium, and one cellulose-based layer containing the active enzymatic system (e.g., glucose oxidase, catalase, glucose, and CaCO3). During the industrial lamination process, the enzyme layer was exposed to three temperature spikes up to 325degreesC without significant enzyme inactivation. Ninety-seven percent of the glucose oxidase activity still remained after the lamination process. The best laminate had an oxygen absorbing capacity of 7.6 +/- 1.0 L/m(2). A reference that was not laminated expressed a corresponding oxygen absorbing capacity of 7.1 +/- 0.8 L/m(2).
  • Andersson, Petra, et al. (författare)
  • Heat Sensing Manikin Test Probe
  • 2000
  • Ingår i: Fire and Materials. - John Wiley & Sons. - 1099-1018. ; 24:4, s. 195-199
  • Tidskriftsartikel (refereegranskat)abstract
    • A manikin test probe has been developed to simulate the thermal behaviour of human skin when exposed to thermal conditions likely to occur in fires. The threshold for second-degree burn injuries is evaluated from the temperature readings using a damage integral. The probe has been exposed to different radiation fluxes and the calculated damage criterion for second-degree burn injuries is in agreement with data from the literature on human skin and pig skin. Probes mounted on a manikin have been used to assess how the extent of burn injuries develop as a function of time on a fully dressed pilot inside a military aeroplane surrounded by a large pool fire. In another scenario burn injuries for humans inside a fire room are assessed when a super-heated water mist extinguishing system is activated.
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